Application of immunohistochemistry and in situ hybridization for detection of bovine coronavirus in paraffin-embedded, formalin-fixed intestines
- 1 November 1997
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 35 (11) , 2964-2965
- https://doi.org/10.1128/jcm.35.11.2964-2965.1997
Abstract
A monoclonal antibody (MAb) (Z3A5) against spike protein subunit of bovine coronavirus (BCV) reacted with the virus in formalin-fixed intestines in an immunoperoxidase test. We found an 88% correlation between immunohistochemistry with Z3A5 and in situ hybridization with a BCV nucleoprotein cDNA probe. MAb Z3A5 reacted with 90 BCV isolates from the United States and was an effective reagent for the diagnosis of BCV.Keywords
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