Abstract
In a preliminary report,1the technic of this suggested test for pregnancy was outlined and the results in fortyfive cases were given. This report deals with ninetyseven additional tests, the results of which, in general, confirm the previous observations. In this communication, three points will be considered: (a) the criteria for the test; (b) the summary of results with mention of special cases, and (c) the application of the test to determine the potency of commercial liquid extracts of ovary, placenta and pituitary. THE CRITERIA OF THE TEST Uterine Enlargement. —In the preliminary investigation, only one criterion was used to indicate a positive test; namely, the enlargement of the uterus of the injected test animal. The total mouse weight divided by the weight of the uterus with ovaries attached gives a ratio which serves as the index. If the ratio is above 400, the test is considered negative for

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