Methylated cap formation by enzymes bound to nuclear informofer particles
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Molecular Biology Reports
- Vol. 4 (2) , 93-96
- https://doi.org/10.1007/bf00775967
Abstract
Rat liver nuclear 30 S ribonucleoprotein particles containing pre-mRNA and nuclear sap proteins have been shown to modify in vitro the synthetic dinucleotide ppGpC in the presence of GTP and S-adenosyl-L-methionine (SAM) by the formation of a blocked and methylated (capped) structure 7meG(5′)ppp(5′)-Gm epC. In the absence of SAM the predominant reaction product was GpppGpC. Our results indicate that the 30S ribonucleoprotein particles (informofers) as well as the proteins of the nuclear sap possess both guanylyltransferase, N7-, and 2-o-methyltransferase activities.Keywords
This publication has 14 references indexed in Scilit:
- Autodegradation of pre-mRNA containing nuclear ribo-nucleoprotein particles. The effect of autodegradation on the double-stranded RNA sequences and on the protein composition of particlesMolecular Biology Reports, 1976
- Kinetics of formation of 5′ terminal caps in mRNACell, 1976
- Methyl labeling of HeLa cell hnRNA: a comparison with mRNACell, 1976
- Protein composition of nuclear 14 S ribonucleoprotein particles containing poly (A)Molecular Biology Reports, 1975
- Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-.Proceedings of the National Academy of Sciences, 1975
- Some Properties of RNA:Protein Complexes from the Nucleus of Eukaryotic CellsCold Spring Harbor Symposia on Quantitative Biology, 1974
- Existence of methylated messenger RNA in mouse L cellsCell, 1974
- A homoribopolynucleotide synthetase in rat liver nuclei associated with ribonucleoprotein particles containing DNA‐like RNAFEBS Letters, 1972
- Cleavage of high-molecular-weight DNA-like RNA by a nuclease present in 30-S ribonucleoprotein particles of rat liver nucleiBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
- The 5′-terminus of bacteriophage R17 RNA: pppGp- - -Journal of Molecular Biology, 1968