The identification of natural and induced bacterial antibody with the serum components in sheep

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Abstract
The electrophoretic pattern and natural agglutinin titer against a range of H antigens of Salmonella were determined in sera from one flock of sheep. The effects of the injection of a bacterial vaccine on the electrophoretic pattern of sheep serum during, and for 12 months after, immunization were studied and showed no characteristic change in pattern. An attempt was made to isolate the natural and induced antibodies by solvent fractionation. Natural antibody to certain Salmonella flagellar antigens was found in fraction, G-2, which was shown electrophoretically to consist of alpha, beta and gamma,-globulins. This antibody did not increase in amount during immunization with an unrelated Salmonella antigen. Induced antibody reached a maximum titer in serum 16-21 days after the first injection of antigen. Antibody, free from natural antibody, was present in the fraction G-3, which was substantially gamma-globulin. In fraction G-2 it was mixed with natural antibody. The isoelectric points of the 2 antibodies appear to be different.
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