Origin and development of protein bodies in cotyledons of Vicia faba
- 1 April 1983
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 157 (5) , 401-410
- https://doi.org/10.1007/bf00397197
Abstract
Storage proteins of the field bean (Vicia faba L., var. minor, cv. “Fribo”) are synthesized and accumulated in the cotyledons during stage 2 of seed development. Deposition of protein reserves takes place in the protein bodies. The generation of protein bodies was investigated electronmicroscopically using ultra-thin sections as well as the freeze-fracturing technique. During the initial period of storage protein formation, globulins are deposited in large vacuoles which later are transformed to give increasing numbers of small vacuoles with decreasing size. The vacuoles disappear early during the stage of storage protein formation and generate the first protein bodies. During the subsequent period of maximum storage protein formation, which takes place at the rough endoplasmic reticulum (rER), swollen ER strands appear which seem to be entirely filled with protein, and these generate ER-produced protein vacuoles (ERPVAC). The vesicles are transformed in a manner comparable to the vacuoles in the initial period of developmental stage 2 and thus generate the major quantity of protein bodies. Both processes seem to represent only two variants of an uniform mechanism of protein body generation.Keywords
This publication has 26 references indexed in Scilit:
- Protein bodies of seeds: Ultrastructure, biochemistry, biosynthesis and degradationPublished by Elsevier ,2001
- Rice protein-body formation: all types are initiated by dilation of the endoplasmic reticulumPlanta, 1982
- Transfer of in vitro synthesized Vicia faba globulins and barley prolamins across the endoplasmic reticulum membrane of Vicia fabaCarlsberg Research Communications, 1981
- Pea seed storage proteins ? Immunocytochemical localization with protein a-gold by electron microscopyProtoplasma, 1981
- Precursor Forms of Pea Vicilin SubunitsPlant Physiology, 1981
- RNA Metabolism and Membrane-Bound Polysomes in Relation to Globulin Biosynthesis in Cotyledons of Developing Field Beans (Vicia fabaL.)European Journal of Biochemistry, 1979
- THE CHEMISTRY AND CELL BIOLOGY OF THE VACUOLAR PROTEINS OF SEEDSJournal of Food Science, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- An agarose mercurial column for the separation of mercaptopapain and nonmercaptopapainBiochimica et Biophysica Acta (BBA) - Protein Structure, 1970
- Protein Bodies of Developing Seeds ofVicia fabaJournal of Experimental Botany, 1969