Interaction of cinnamyl‐tRNAPhe with Escherichia coli elongation factor Tu
Open Access
- 10 January 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 151 (1) , 143-147
- https://doi.org/10.1016/0014-5793(83)80360-3
Abstract
The products of nitrous acid mediated‐deamination of Phe‐tRNAPhe from E. coli were analyzed and their capability to interact with elongation factor Tu from E. coli was investigated. Thin‐layer chromatography as well as HPLC analysis revealed the existence of at least two deamination products, 3‐phenyllactyl‐tRNAPhe and cinnamyl‐tRNAPhe. It could be shown that the aminoacyl‐tRNA analogues were active in the formation of the ternary complex with EF‐Tu·GTP, although with a lower efficiency than native Phe‐tRNAPhe. For both modified acyl‐tRNAs the dissociation constant was determined to be 3 × 10−5 M.Keywords
This publication has 14 references indexed in Scilit:
- On the Properties of Immobilized Elongation Factor Tu from Thermus thermophilus HB8European Journal of Biochemistry, 1982
- A simplified procedure for the isolation of bacterial polypeptide elongation factor EF-TuAnalytical Biochemistry, 1980
- Participation of X47-fluorescamine modified E. coli tRNAs in in vitro protein biosynthesisNucleic Acids Research, 1978
- Specificity of Elongation Factor Tu from Escherichia coli with Respect to Attachment of the Amino Acid to the 2' or 3'-Hydroxyl Group of the Terminal Adenosine of tRNAEuropean Journal of Biochemistry, 1977
- 3-(3-Amino-3-carboxypropyl)uridine. Novel modified nucleoside isolated from Escherichia coli phenylalanine transfer ribonucleic acidBiochemistry, 1974
- Formation of a ternary complex of phenyllactyl-tRNA with transfer factor Tu and GTPBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- Neighboring aryl group participation in nitrous acid deaminations of l-phenylalanine ethyl ester and its p-nitro and p-methoxy derivativesTetrahedron Letters, 1971
- Specificity of the interaction of aminoacyl ribonucleic acid with a protein-guanosine triphosphate complex from wheat embryoBiochemistry, 1969
- Evidence for a guanine nucleotide-aminoacyl-RNA complex as an intermediate in the enzymatic transfer of aminoacyl-RNA to ribosomesBiochemical and Biophysical Research Communications, 1967
- Incorporation of phenyllactic acid in terminal position during polyphenylalanine biosynthesisJournal of Molecular Biology, 1965