Nutrient Requirements for the Recovery of Tellurite Resistance by Heat-injured Streptococcus faecalis

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Abstract
Heat-injured S. faecalis recovered tellurite resistance in 4-5 h when incubated at 33.degree. C in a non-growth medium containing KH2PO4/K2HPO4 buffer pH 7.1, glucose and casein hydrolysate. Only 10% of the damaged population recovered in the absence of K+, or glucose, or casein hydrolysate, or in the presence of valinomycin in medium containing 1 mM K+. The K+ content of cells increased 2- to 3-fold on heating and a further 3- to 6-fold during the first 30 min of subsequent incubation. The Na+ content decreased simultaneously but Mg2+ concentrations were not affected. Recovery was not affected by adding 1 mM EDTA or 2 mM Mg2+ to the medium but the addition of 1 mM EDTA and 2 mM Mg2+ together reduced recovery to < 20%. This effect reversed by adding 2 mM Ca2+.

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