The role of protein kinase activation in the control of steroidogenesis by adrenocorticotrophic hormone in the adrenal cortex
- 1 December 1973
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 136 (4) , 993-998
- https://doi.org/10.1042/bj1360993
Abstract
A method has been developed for investigation of the effect of adrenocorticotrophic hormone (ACTH) on the state of activation of a cyclic AMP-dependent protein kinase within cells of the adrenal cortex. Enzyme activity was measured in terms of the quantity of32P transferred from [γ-32P]ATP to histone under conditions in which bound cyclic AMP did not dissociate from the regulatory subunit of the protein kinase ACTH (1×10-2i.u./ml) caused a rapid and complete activation of the cyclic AMP-dependent protein kinase activity within 2min of hormone addition to the isolated cells. In response to a range of ACTH concentrations a sigmoid log dose–response curve for protein kinase activation was obtained, with half-maximal stimulation attained at about 1×10-3i.u./ml. However, some low doses of ACTH that elicited a marked (but submaximal) steroidogenic response failed to cause a clear stimulation of protein kinase activity in isolated adrenal cells. Theophylline (2mm) potentiated the effect of ACTH on protein kinase activity. The results implicate an important role for protein kinase in ACTH action on the adrenocortical cell.Keywords
This publication has 20 references indexed in Scilit:
- Comparison of Steroidogenic Effects of cAMP and dbcAMP in the Rat Adrenal Gland1 1Endocrinology, 1973
- Phosphorylation of an Endogenous Membrane Protein by an Endogenous, Membrane-associated Cyclic Adenosine 3′,5′-Monophosphate-dependent Protein Kinase in Human Erythrocyte GhostsJournal of Biological Chemistry, 1972
- Cyclic Adenosine 3′,5′-Monophosphate-dependent Protein Kinase of Human Erythrocyte MembranesJournal of Biological Chemistry, 1972
- CORTICOSTEROIDOGENESIS IN ISOLATED ADRENAL CELLS: EFFECT OF ADRENOCORTICOTROPHIC HORMONE, ADENOSINE 3′,5′-MONOPHOSPHATE AND β1–24ADRENOCORTICOTROPHIC HORMONE DIAZOTIZED TO POLYACRYLAMIDEJournal of Endocrinology, 1972
- Isolated adrenal cells: Steroidogenesis and cyclic AMP accumulation in response to ACTHArchives of Biochemistry and Biophysics, 1972
- Interaction with phosphodiesterase of free and kinase-complexed cyclic adenosine 3',5'-monophosphate.1971
- Role of the Receptor in the Mechanism of Action of Adenosine 3′:5′-Cyclic MonophosphateProceedings of the National Academy of Sciences, 1971
- A cyclic-3′,5′-adenosine monophosphate dependent protein kinase from the adrenal cortex: Comparison with a cyclic AMP binding proteinBiochemical and Biophysical Research Communications, 1970
- An Adenosine 3′,5′-Monophosphate-dependant Protein Kinase from Rabbit Skeletal MuscleJournal of Biological Chemistry, 1968
- Adenosine 3‘,5‘-Monophosphate as the Intracellular Mediator of the Action of Adrenocorticotropic Hormone on the Adrenal CortexJournal of Biological Chemistry, 1967