Structural and Serological Characterisation of the O‐Specific Polysaccharide from Lipopolysaccharide of Acinetobacter calcoaceticus Strain 7 (DNA Group 1)
Open Access
- 1 January 1997
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 243 (1-2) , 167-173
- https://doi.org/10.1111/j.1432-1033.1997.0167a.x
Abstract
S‐form lipopolysaccharide was isolated by phenol/water extraction from a strain of Acinetobacter calcoaceticus (DNA group 1). The structure of the O‐antigenic polysaccharide was determined by compositional analysis and NMR spectroscopy of the de‐O‐acylated lipopolysaccharide. The isolated polysaccharide obtained after hydrolysis of lipopolysaccharide in 0.01 M trifluoroacetic acid has the following structure: in which Pyr is pyruvate. The O‐acetyl substitution of D‐Gal was non‐stoichiometric. The O‐antigen was specifically recognised in western blots by polyclonal rabbit antisera.Keywords
This publication has 23 references indexed in Scilit:
- Structural and Serological Characterisation of Two O‐Specific Polysaccharides of AcinetobacterEuropean Journal of Biochemistry, 1996
- Bacteremia due toAcinetobacter species other thanAcinetobacter baumanniiInfection, 1994
- The structure of the O‐specific chain of Legionella pneumophila serogroup 1 lipopolysaccharideEuropean Journal of Biochemistry, 1994
- The structure of the O‐antigenic polysaccharide from lipopolysaccharide of Vibrio cholerae strain H11 (non‐O1)European Journal of Biochemistry, 1992
- Outer membrane vesiculation of acinetobacter calcoaceticusActa Biotechnologica, 1990
- Effect of plasmid RP1 on phase changes in inner and outer membranes and lipopolysaccharide from Acinetobacter calcoaceticus: a Fourier transform infrared studyBiochemistry, 1987
- Studies on the chemical structure of the core-lipid A region of the lipopolysaccharide of Acinetobacter calcoaceticus NCTC 10305. Detection of a new 2-octulosonic acid interlinking the core oligosaccharide and lipid A componentEuropean Journal of Biochemistry, 1987
- Identification of 2‐keto‐3‐deoxy‐1,7‐dicarboxyheptonic acid as a constituent of the lipopolysaccharide of Acinetobacter calcoaceticus NCTC 10305European Journal of Biochemistry, 1985
- Structural determination of the capsular polysaccharide of Neisseria meningitidis group I: a two-dimensional NMR analysisBiochemistry, 1985
- Isolation, Purification, and Chemical Analysis of the Lipopolysaccharide Analysis of the Lipopolysaccharide and Lipid A of Acinetobacter calcoaceticus NCTC 10305European Journal of Biochemistry, 1982