Electron Transport-Linked Compared with Proton-Induced ATP Generation in Thiobacillus novellus
- 1 December 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (12) , 3571-3575
- https://doi.org/10.1073/pnas.70.12.3571
Abstract
The apparently soluble electron-transport system, that does not sediment when centrifuged at 144,000 x g or 300,000 x g for 3 hr, catalyzes oxidative phosphorylation with an efficiency comparable to that of an intact mitochondrial system. While the proton-induced phosphorylation occurs in whole cells, crude cell-free extracts, and supernatants from low-speed centrifugation, it does not occur in either the 144,000 or 300,000 x g supernatant fractions. The data show that oxidative energy can be conserved as ATP under conditions that are incompatible with the basic postulate of the chemiosmotic hypothesis.Keywords
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