The response of leucocytes of agammaglobulinaemia subjects to phythohaemagglutinin and anti-immunoglobulin antiserum.
- 1 February 1969
- journal article
- Vol. 4 (2) , 203-11
Abstract
Leucocytes of three patients with congenital (case 1) or acquired (cases 2 and 3) agammaglobulinaemia were studied in vitro in cell culture for their capacity to undergo blastogenesis and mitosis upon exposure to phytohaemagglutinin (PHA) or anti-immunoglobulin sera. Synthesis of DNA was measured by the incorporation of tritiated thymidine added 16 hr before termination of culture. The lymphocytes of patients Nos. 2 and 3 showed little or no uptake of the labelled thymidine following stimulation by antisera, whereas those of patient No. 1 were at the upper limit of normal. Response to stimulation by PHA was within the normal range for patients Nos. 1 and 3 but subnormal for patient No. 2. The different responses to PHA and anti-immunoglobulin antisera suggest basic heterogeneity of the defects in the lymphocytes of these patients.This publication has 17 references indexed in Scilit:
- CHRONIC MUCOCUTANEOUS CANDIDIASIS, DEFICIENCY OF DELAYED: HYPERSENSITIVITY, AND SELECTIVE LOCAL ANTIBODY DEFECTThe Lancet, 1967
- The production of immunoglobulins by human peripheral blood lymphocytes in vitro.1967
- QUANTITATIVE STUDIES OF PHYTOHEMAGGLUTININ-INDUCED DNA AND RNA SYNTHESIS IN NORMAL AND AGAMMAGLOBULINEMIC LEUKOCYTESThe Journal of Experimental Medicine, 1967
- Studies on human lymphocytes stimulated in vitro with anti-γ and anti-μ antibodiesCellular and Molecular Life Sciences, 1967
- The activation and reactivation of peripheral lymphocytes in cultureJournal of Cell Science, 1967
- STUDIES ON RABBIT LYMPHOCYTES IN VITROThe Journal of Experimental Medicine, 1967
- Role of the Thymus and Other Central Lymphoid Tissues in Immunological DiseaseAnnual Review of Medicine, 1967
- STUDIES ON RABBIT LYMPHOCYTES IN VITROThe Journal of Experimental Medicine, 1967
- STUDIES ON RABBIT LYMPHOCYTES IN VITROThe Journal of Experimental Medicine, 1965
- The chemistry of certain imines related to the diterpene alkaloidsCellular and Molecular Life Sciences, 1964