In vitro perfused, non-isolated small intestine: Ontogeny of transmural hydraulic permeability

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Abstract
Net fluid-flux and transmural hydrostatic hydraulic conductance (Lp) were measured in the rat ileum during postnatal ontogeny, utilizing a refined in vitro, capillary-perfused (non-isolated) preparation for free-flow studies. Volume absorption at low transmural pressure gradient (1–2 cmH2O) was 0.99±0.22 ml/g wet weight·hr in the mature ileum and 1.55±0.42 ml/g wwt·hr in the differentiating intestine. Hydrostatic hydraulic conductance of the mature ileum was 0.76 μl/cm2·cm H2O·hr, indicating that normal hydrostatic pressure gradients are not important for modulation of intestinal absorption. In contrast, Lp was 3.82 μl/cm2·cmH2O·hr in the differentiating ileum, suggesting a significant contribution of pressure-dependent solvent drag to isotonic solute absorption.

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