Structure of Horseradish Perosidase Compound I
- 1 April 1983
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 132 (1) , 147-150
- https://doi.org/10.1111/j.1432-1033.1983.tb07339.x
Abstract
The kinetics of the reaction between horseradish peroxidase and p-nitroperbenzoic acid to form compound I were studied at 25.degree. C in phosphate buffer pH 7.2 and ionic strength of 0.11 M by transient-state and steady-state methods. The 2nd-order rate constant for compound I formation obtained by stopped-flow measurements at 403 nm is (3.7 .+-. 0.2) .times. 107 M-1 s-1. For the disappearance of p-nitroperbenzoic acid and appearance of p-nitrobenzoic acid using steady-state kinetics measured at 265 nm the rate constant is (3.0 .+-. 0.6) .times. 107 M-1 s-1. The results provide an independent confirmation that one, and only 1, oxygen atom is incorporated from the oxidizing substrate into compound I.This publication has 20 references indexed in Scilit:
- Heterogeneous one-electron reduction of metal-containing biological molecules using molecular hydrogen as the reductant: synthesis and use of a surface-confined viologen redox mediator that equilibrates with hydrogenJournal of the American Chemical Society, 1981
- Meaning of diffusion-controlled association rate constants in enzymologyThe Journal of Physical Chemistry, 1979
- Proton balance in conversions between five oxidation-reduction states of horseradish peroxidaseArchives of Biochemistry and Biophysics, 1974
- The substrate profiles of the acidic and slightly basic horseradish peroxidasesBiochimica et Biophysica Acta (BBA) - Enzymology, 1974
- Biochemical significance of porphyrin .pi. cation radicalsAccounts of Chemical Research, 1974
- Studies on Horseradish Peroxidase. XI. On the Nature of Compounds I and II as Determined from the Kinetics of the Oxidation of FerrocyanideCanadian Journal of Chemistry, 1973
- Studies on Horseradish Peroxidase. VII. A Kinetic Study of the Oxidation of Iodide by Horseradish Peroxidase Compound IICanadian Journal of Chemistry, 1971
- Compounds I of Catalase and Horse Radish Peroxidase: π-Cation RadicalsProceedings of the National Academy of Sciences, 1971
- Primary compounds of catalase and peroxidaseBiochemical Journal, 1961
- Intermediate Compounds Formed when Horseradish Peroxidase Reacts with Potassium Molybdicyanide and Potassium ChloriridateScience, 1953