Mode of Inhibition of the Aspergillus Amine Oxidase by 8-Hydroxyquinoline
- 1 October 1972
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 72 (4) , 833-839
- https://doi.org/10.1093/oxfordjournals.jbchem.a129977
Abstract
The inhibitory effect of 8-hydroxyquinoline on the amine oxidase [monoamine: oxygen oxidoreductase (deaminating), EC 1. 4. 3. 4] from Aspergillus niger was analyzed on the basis of data which were obtained by the overall reaction kinetics and also by the “flow” method. It was confirmed that 8-hydroxyquinoline inhibited the trans-amination step non-competitively, but did not inhibit the oxidative reaction of the pyridoxamine phosphate (PMP) form with molecular oxygen. The following reaction scheme for the mode of inhibition of the amine oxidase by 8-hydroxyquinoline was proposed: where EL stands for the pyridoxal phosphate (PLP) form of the amine oxidase (enzyme unit based on PLP), EM for the PMP form and X and Y for intermediates in the reactions. ELI and XI are a PLP form-inhibitor complex and an intermediate (X)-inhibitor complex(es),** respectively.Keywords
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