Identification of two macrophage populations by flow cytometry monitoring of oxidative burst and phagocytic functions
- 1 January 1986
- journal article
- research article
- Published by Wiley in Biology of the Cell
- Vol. 57 (2) , 143-146
- https://doi.org/10.1111/j.1768-322x.1986.tb00470.x
Abstract
Two populations of phagocytic cells from trehalose dimycolate-elicited mouse peritoneal cells are demonstrated by flow cytofluorometry, using two fluorescent probes excited at the same wavelength (488 nm). Liposomes containing diethylenetriaminepentaacetate daunorubicin conjugate (maximum emission wavelength: 590 nm) allow the discrimination of phagocytes and non-phagocytic cells. Among the phagocytes, an activated population is revealed by a cell-associated fluorescence of the oxidation product of dichlorofluorescein diacetate (maximum emission wavelength: 520 nm).This publication has 0 references indexed in Scilit: