Abstract
A laboratory colony of Leptotrombidium arenicola infected with Rickettsia tsutsugamushi, the etiologic agent of scrub typhus, was derived from a single field-collected mite. Transovarial transmission and filial infection rates were determined by single feedings of 906 larvae from 51 individual infected females. Through 4 generations, the transovarial transmission rate was 100% and the filial infection rates varied from 20% to 100% with an overall rate of 97%. The significance of these findings in scrub typhus epidemiology is discussed.

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