Studies on C19 Steroid Metabolism in American Bullfrog (Rana catesbeiana) Adrenal Mitochondria
- 1 April 1972
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 50 (4) , 443-446
- https://doi.org/10.1139/o72-060
Abstract
'Mitochondrial' preparations from adrenals of the American bullfrog (Rana catesbeiana) were incubated with 14C-labelled androstenedione (Δ4A) and testosterone (T) as model substrates in the presence of an NADPH-generating system. The conversion of Δ4A to 11β-OH-Δ4A and T was 1.4% and 0.8%, respectively. T was metabolized to 11β-OH-Δ4A (1.7%), 11β-OH-T (0.9%), and Δ4A (3.3%). All the above-mentioned metabolites were identified by isotope dilution techniques. From these results it appears that bullfrog adrenal 'mitochondria' possess 17β-hydroxysteroid oxidoreductase, though the adrenal tissue itself lacks 17α-hydroxy-lase activity.Keywords
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