Selection and validation of a set of reliable reference genes for quantitative sod gene expression analysis in C. elegans
Open Access
- 22 January 2008
- journal article
- research article
- Published by Springer Nature in BMC Molecular Biology
- Vol. 9 (1) , 9
- https://doi.org/10.1186/1471-2199-9-9
Abstract
In the nematode Caenorhabditis elegans the conserved Ins/IGF-1 signaling pathway regulates many biological processes including life span, stress response, dauer diapause and metabolism. Detection of differentially expressed genes may contribute to a better understanding of the mechanism by which the Ins/IGF-1 signaling pathway regulates these processes. Appropriate normalization is an essential prerequisite for obtaining accurate and reproducible quantification of gene expression levels. The aim of this study was to establish a reliable set of reference genes for gene expression analysis in C. elegans.Keywords
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