Selective excretion of alkaline xylanase by Escherichia coli carrying pCX311.
- 1 January 1985
- journal article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 49 (10) , 3011-3015
- https://doi.org/10.1271/bbb1961.49.3011
Abstract
Plasmid pCX311, which we constructed, has two HindIII DNA fragments (2.6kbp and 2.Qkbp) of alkalophilic Bacillus sp. strain C-125 in the HindIII site of pBR322. These two fragments were essential not only for the xylanase production but also for the excretion of periplasmic proteins. The cloned 4.6 kbp fragment encodes some components that made the outer membrane of E. coli permeable. Some proteins such as xylanase and β-lactamase were excreted, but alkaline phosphatase was not excreted into the culture broth.Keywords
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