Narrow (5‐50‐μm i.d.) open tubular columns in liquid chromatography using immobilized polymethyloctadecylsiloxane as stationary phase

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Abstract
This paper presents in detail the preparation of highly efficient, stable open tubular (OT) columns for reversed‐phase liquid chromatography. Chromatographic properties of 5‐50‐μm i.d. fused silica columns (1.5‐5 m in length) are described, and details of the coating procedure are discussed. On‐column fluorescence detection was used for the model substituted‐anthracene test solutes. Gas chromatography was used as a complementary technique for column characterization.The stationary phase studied, immobilized polymethyloctadecylsiloxane, PMSC18, was chosen because of its more lipophilic character compared to other siloxanes. As expected, a higher retention power was found compared to polydimethylsiloxane coatings.The polymeric stationary phase showed good properties. More than 900,000 plates were achieved in 25 min (k = 0.1) in a 5‐μm i.d. fused silica OT column. The stationary phase contribution to band broadening was less than 10% in an 11‐μm i.d. column at a flow rate thirty times uopt (k = 1.3).

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