Practical Approach for Detection and Identification of OXA-10-Derived Ceftazidime-Hydrolyzing Extended-Spectrum β-Lactamases
- 1 March 1998
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 36 (3) , 827-829
- https://doi.org/10.1128/jcm.36.3.827-829.1998
Abstract
A practical approach to detect and identify ceftazidime-hydrolyzing extended-spectrum mutants of OXA-10 β-lactamase is presented. Large numbers of bacteria were screened by colony hybridization, a 720-bp part of blaOXA was amplified by PCR from the hybridization-positive isolates, and the products were digested byPvuII and HaeIII.Keywords
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