STUDIES OF THE MITOTIC FIGURE

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Abstract
The method is the same as the author has previously described (see B. A. 8: entry 133) in a study of the effects of modifications of a single fixing fluid on the eggs of C. pergamentaceus. 116 formulae given fall into the following groups: (1) standard fixatives ; (2) "Original reagents"; (3) components of above fixatives, separately; and (4) dilutions of 1, 2 and 3. The percentage by weight of each component is given. Each reagent is judge''d by its effect on (a) the central body and astral rays and (b) on the general cytoplasmic configuration. Centrioles are demonstrated by only 4 of the fixatives, each of which contained one or more of the following: picric acid, acetic acid, formaldehyde and alcohol. Acetic acid was the only component present in all 4. The character of the work may best be judged by the author''s own words: "After the use of all reagents the eggs are extensively shrunken. Those which are about 40% of the volume of the living egg have un-condensed centers; those shrunk to about 30% have condensed ones and some of these contain centrioles." The conclusion is that "The data of the present study are in harmony with those of the previous one." [See also B. A. 7: Entry 20267.].

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