Standardization of Serum Ceruloplasmin Concentrations in International Enzyme Units with o-Dianisidine Dihydrochloride as Substrate
- 1 December 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 20 (12) , 1564-1567
- https://doi.org/10.1093/clinchem/20.12.1564
Abstract
We describe a method for calculating the absorptivity (in terms of substrate consumed) of the colored solution obtained when o-dianisidine dihydrochloride is oxidized by ceruloplasmin. By oxidizing o-dianisidine dihydrochloride with known amounts of hydrogen peroxide we could determine that the molar reacting ratio of o-dianisidine to hydrogen peroxide is 2:1. So calculated, absorptivity is 9.6 ml µmol-1 cm-1, the figure used to estimate ceruloplasmin oxidase activity in terms of International Units.Keywords
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