The preparation and characterization of vitellogenin messenger RNA from rainbow trout (Salmo gairdneri)

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Abstract
Agarose-gel electrophoresis of polyARNA from livers of estrogen-treated male rainbow trout revealed a major high-MW species (7200 nucleotides), which is absent from the polyARNA isolated from hormonally unstimulated male trout liver. Translation in vitro of the RNA from estrogen-treated males in a mRNA-dependent rabbit reticulocyte lysate produced a protein (MW 200,000) that could be immunoprecipitated with antibodies against trout serum vitellogenin, but no immunoprecipitable protein was synthesized with RNA from control animals. DNA complementary to the RNA from estrogen-stimulated and control male trout liver was synthesized and back-hybridized, with R0t1/2 [RNA concentration .times. incubation time needed to complete 1/2 of hybridization] of 3.8 .times. 10-2 and 1 .times. 10-1 mol .cntdot. l-1 .cntdot. s for RNA from hormone-treated and control animals, respectively. The 9% increase in the abundant mRNA after estrogen stimulation is due to the induction of vitellogenin mRNA.