Nonprecipitating and Electrophoretically Restricted Antibodies to Carbohydrate of Group A Streptococcus in the Rat

Abstract
Hyperimmunization of Sprague-Dawley rats with group A streptococcal vaccine elicited both precipitating and nonprecipitating antibodies to the group-specific carbohydrate of the Streptococcus. The occurrence of nonprecipitating antibodies in the immune response of the rat appeared to be genetically influenced by selective inbreeding that causes a low precipitin response. A number of rats responded to immunization by producing electrophoretically restricted antibody components. This restriction in light-chain heterogeneity confirmed by polyacrylamide gel electrophoresis of isolated antibody is apparently genetically influenced and capable of recall after extended periods of nonimmunization.

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