The possible significance of hexosephosphoric esters in ossification

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Abstract
Calcification was obtained at lower levels of the product (Ca) X (P) when the concentration of Ca was high and that of phosphate low than when the conditions were reversed. Bicarbonate was favorable to the deposition of Ca. KC1 in concentrations equal to twice that of plasma did not inhibit calcification in inorganic solutions or in the presence of phosphoric ester. Mg, in concentrations as low as 4 mgm. per 100 cc., inhibited calcification in inorganic solutions but this effect was less evident when phosphoric ester was present. The in- organic solutions in which calcification took place were supersaturated with respect to the Ca salt deposited. Between pH 7.2 and 8.0 there was no optimum either in the presence or absence of ester. Formaldehyde inhibited calcification in inorganic solutions as well as in the presence of ester. Chloroform and KCN both inhibited calcification in inorganic solutions but had no apparent effect in the presence of the ester. Bone slices, which were treated with organic solvents, or desiccated in vacuo over H2SO4, failed to calcify when placed in solutions of high (Ca)X(P) levels but calcified in solutions of phosphoric ester.