Carotenoid biosynthesis by Aphanocapsa homogenates coupled to a phytoene-generating system from Phycomyces blakesleeanus

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Abstract
The ability of a cell extract from the C5 carB10(-) (phytoene-accumulating) strain of Phycomyces blakesleeanus to convert [2-14C]mevalonic acid into phytoene has been exploited in a coupled assay with a cell homogenate from the cyanobacterium Aphanocapsa 6714 (Pasteur Culture Collection]. Incubations containing both cell extracts convert [2-14C]mevalonic acid into unsaturated carotenes and xanthophylls of Aphanocapsa, via the [14C]phytoene formed by the C5 preparation. The major advantage of this experimental approach is that it simplifies and shortens the assay procedure, since it eliminates the necessity to initially prepare [14C]phytoene and then add it to the incubation with an emulsifying agent.