Effects of hypophysectomy and growth hormone on utilizable amino acid accumulation

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Abstract
Hypophysectomy decreased the concentrations of alanine and histidine in the cell water of the rat diaphragm, but had no influence on the levels of these amino acids in plasma. The concentrations of alanine and histidine in the cell water did not vary appreciably from in vivo values, when "intact" diaphragms of either normal or hypophysectomized rats were incubated in Krebs bicarbonate buffer. Addition of l-alanine or l-histidine to the incubation medium resulted in the accumulation of these amino acids in the cell water of both normal and hypophysectomized rat diaphragms. However, muscles or hypophysectomized rats accumulated these amino acids to a lesser extent. When added to the incubation medium, bovine growth hormone stimulated the accumulation of l-alanine and l-histidine by diaphragms of hypophysectomized rats. Thus, these experiments suggest that growth hormone participates in the regulation of utilizable amino acid transport into muscle.