The induction of the human T‐cell growth factor receptor precedes the production of RNA and occurs in the presence of inhibitors of RNA synthesis

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Abstract
The receptor for T‐cell growth factor (TCGF) is an activation antigen that is present in low amounts on a small fraction of resting T lymphocytes. The TCGF receptor on human T cells can be detected with the anti‐Tac monoclonal antibody within 7–12 h of stimulating the cells with phytohemagglutinin (PHA). In the current studies, we examined human lymphocytes cultured alone, with PHA, or with PHA plus sufficient actinomycin‐D to inhibit RNA synthesis. After varying intervals, aliquots of the lymphocytes were stained with acridine orange (AO) or pyronin‐Y(PY) to measure RNA and/or with anti‐Tac plus FITC goat anti‐mouse lg. Tac expression began to increase after 6–8 h incubation with PHA, whereas increases in PY or AO staining were not detected until 12 h or later. Furthermore, the initial increase in Tac expression was not affected by sufficient actinomycin‐D to block all detectable nucleic acid synthesis. Therefore, it appears that the initial expression of TCGF receptors detected after lymphocyte activation does not require de novo production of RNA.

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