Purification and amino acid sequence of two small, acid-soluble proteins from Clostridium bifermentans spores

Abstract
Clostridium bifermentans spores contain two major small, acid-soluble, proteins (SASP) termed SASP-α and β. The amino acid sequences of SASP-α and β are almost identical, and are very similar to those of α/β-type SASP from spores of C. perfringens and various Bacillus species. However, the C. bifermentans proteins contain an extra five amino acids in the middle of their sequence. Surprisingly, no γ-type SASP were found in C. bifermentans or C. perfringens spores, although these are the most prominent SASP in spores of Bacillus species.

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