EVALUATION OF ANTITHYMOCYTE GLOBULIN FOR HUMAN BONE MARROW TRANSPLANTATION

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Abstract
Antihematopoietic stem cell activity and immunosuppressive activity were evaluated before and after absorption of horse antihuman thymocyte globulin (ATG) with mature [human] granulocytes and fetal liver cells. Unabsorbed ATG was cytotoxic to committed myeloid-macrophage progenitors (CFU-C). Absorption of ATG removed cytotoxicity to CFU-C. The immunosuppressive potentials of absorbed ATG were identical to those of unabsorbed ATG in several tests. Primary response to trinitrophenyl-modified autologous and allogeneic cells could be suppressed by treatment with ATG alone whereas suppression of secondary and cytotoxic responses required treatment with ATG and complement. Unsensitized or undifferentiated T cells may be more sensitive to ATG than are sensitized or differentiated T cells.