Growth and Differentiation of Plant Tissue Cultures

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Abstract
Explants derived from artichoke tubers proliferate rapidly when cultured aseptically on a nutrient medium containing sugar, mineral salts, coconut milk, and 2, 4 dichlorophenoxyacetic acid. This rapid growth, which occurs in three distinct stages, is accompanied by massive changes in numbers of cells, fresh and dry weight, and pronounced changes in the rates of gaseous exchange. The very short lag phase is followed by a period of exponential cell division during which the original cellular pattern is partially obliterated. At the end of this growth phase redifferentiation occurs, tracheidea are formed in large numbers, and distinctive nodular bodies appear which resemble meristems. Under the conditions of these experiments they do not develop further.

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