Characterisation of theARG7gene ofChlamydomonas reinhardtiiand its application to nuclear transformation
Open Access
- 1 May 1995
- journal article
- research article
- Published by Taylor & Francis in European Journal of Phycology
- Vol. 30 (2) , 141-148
- https://doi.org/10.1080/09670269500650901
Abstract
The Chlamydomonas reinhardtii gene ARG7 encoding the enzyme argininosuccinate lyase is a popular marker for the nuclear transformation of this alga. We present the complete nucleotide sequence of ARG7. The gene spans 7 kilobases and is split by 12 introns containing highly repetitive microsatellite elements. Molecular analysis of eight different arg7 mutants indicates that none of the mutations is the result of gross rearrangements at the ARG7 locus. We describe our analysis of factors influencing transformation efficiency and the construction of a cosmid library of the Chlamydomonas genome in a customised vector carrying the ARG7 marker. This library should greatly facilitate the cloning of Chlamydomonas genes by complementation.Keywords
This publication has 32 references indexed in Scilit:
- Mutants of Chlamydomonas with Aberrant Responses to Sulfur Deprivation.Plant Cell, 1994
- Insertional mutagenesis to isolate acetate-requiring mutants inChlamydomonas reinhardtiiFEMS Microbiology Letters, 1993
- Expression of chimeric genes by the light-regulated cabII-1 promoter in Chlamydomonas reinhardtii: a cabII-1/nit1 gene functions as a dominant selectable marker in a nit1- nit2- strain.Molecular and Cellular Biology, 1992
- Expression of the arylsulfatase gene from theβ2-tubulin promoter inChlamydomonas reinhardtiiNucleic Acids Research, 1992
- PHYLOGENY OF CHLAMYDOMONAS (CHLOROPHYTA) BASED ON CLADISTIC ANALYSIS OF NUCLEAR 18S rRNA SEQUENCE DATA1Journal of Phycology, 1990
- Studies on the maintenance and expression of cloned DNA fragments in the nuclear genome of the green alga Chlamydomonas ReinhardtiiPhysiologia Plantarum, 1990
- Nucleotide sequences of two genesCAH1andCAH2which encode carbonic anhydrase polypeptides inChlamydomonas reinhardtiiNucleic Acids Research, 1990
- Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii.Proceedings of the National Academy of Sciences, 1989
- Characterization of argininosuccinate lyase (EC 4.3.2.1) from Chlamydomonas reinhardtiiBiochemical Journal, 1987
- Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtiiJournal of Molecular Biology, 1986