HIGH-RESOLUTION PREPARATIVE COLUMN CHROMATOGRAPHIC SYSTEM FOR GANGLIOSIDES USING DEAE-SEPHADEX AND A NEW PORUS SILICA, IATROBEADS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 441  (3) , 488-497
Abstract
A new high-resolution preparative column chromatographic system was developed for efficient and rapid isolation of ganglioside molecular species. The system involved a combination of ion-exchange and adsorption chromatographies using DEAE-Sephadex A-25 and the newly developed, totally porous silica spheres, Iatrobeads. Using this system the bovine brain gangliosides, GM1, GD1a, GD1b and GT1 were obtained in high purity and in milligram amounts, in a relatively short time, and by simple procedures. The presence of a number of unidentified molecular species of gangliosides, which are present only in small amounts, was also demonstrated.

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