Identification of 24,25,26,27-tetranor-23-hydroxyvitamin D3 as a product of the renal metabolism of 24,25-dihydroxyvitamin D3
- 31 July 1984
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 23 (16) , 3749-3754
- https://doi.org/10.1021/bi00311a028
Abstract
By cochromatography, mass spectrometry and chemical derivatization, it was shown that a metabolite isolated from the perfused rat kidney incubated with 24-(R),25-dihydroxyvitamin D3 is indistinguishable from chemically synthesized 24,25,26,27-tetranor-23-hydroxyvitamin D3. The new metabolite is also produced from 24-oxo-25-hydroxyvitamin D3 but not from 23(S),25-dihydroxyvitamin D3. Enzymes required for the synthesis of the new metabolite are absent in the vitamin D deplete animal but are induced along with the 25-hydroxyvitamin-D3 24-hydroxylase by vitamin D repletion. The pathway of 24,25-dihydroxyvitamin treatment of the rat with large doses of vitamin D3, suggesting that the pathway is a degradative one.This publication has 1 reference indexed in Scilit:
- 25-hydroxyvitamin D3 metabolism by isolated perfused rat kidneyAmerican Journal of Physiology-Endocrinology and Metabolism, 1980