Molekularbiologische Identifizierung von Cunninghamella spec.
- 1 September 2002
- Vol. 45 (S1) , 31-36
- https://doi.org/10.1111/j.1439-0507.2002.tb04543.x
Abstract
Among the genus Cunninghamella, so far C. bertholletiae is known to be the only clinically relevant species. Correct identification of C. bertholletiae is not possible with classical methods. PCR and sequencing of the internal transcribed spacer (ITS) region was used to identify seven of nine clinical isolates as C. bertholletiae and two as C. echinulata. Also an isolate of the surrounding area of one patient infected with C. echinulata could be identified as C. echinulata. High homology in the ITS region was found within the isolates of C. bertholletiae. Within the species C. echinulata and C. elegans a differentiation on subspecies level was achieved by an analysis of restriction fragment length polymorphism of the ITS amplicons after incubation with TaqI and HinfI. Similar results were obtained by PCR fingerprinting of the complete DNA with the single microsatellite DNA primers (GTG)5 and (GAC)5. For the first time C. echinulata could be identified as agent of zygomycosis in humans.Keywords
This publication has 13 references indexed in Scilit:
- A case of imported paracoccidioidomycosis in a German legionnaireMedical Mycology, 2002
- Disseminated Neocosmospora vasinfecta Infection in a Patient with Acute Nonlymphocytic LeukemiaEmerging Infectious Diseases, 2001
- Cluster of Pulmonary Infections Caused byCunninghamella bertholletiaein Immunocompromised PatientsClinical Infectious Diseases, 2000
- RFLP analysis reveals marked geographical isolation between strains ofParacoccidioides brasiliensisMedical Mycology, 2000
- Cunninghamella bertholletiae Infection Mimicking Myocardial InfarctionClinical Infectious Diseases, 1999
- Systematic relationship of several controversial Cunninghamella taxa inferred from sequence comparisons of ITS2 of rDNAMycological Research, 1999
- Polymerase chain reaction fingerprinting in fungi using single primers specific to minisatellites and simple repetitive DNA sequences: Strain variation in Cryptococcus neoformansElectrophoresis, 1995
- A rare zygomycosis due to Cunninghamella bertholletiaeMycoses, 1993
- The case for Cunninghamella elegans, C. bertholletiae and C. echinulata as separate speciesTransactions of the British Mycological Society, 1984
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977