AGGREGATION OF COLLAGEN EXPOSED TO UVA IN THE PRESENCE OF RIBOFLAVIN: A PLAUSIBLE ROLE OF TYROSINE MODIFICATION
- 1 March 1994
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 59 (3) , 343-349
- https://doi.org/10.1111/j.1751-1097.1994.tb05045.x
Abstract
Riboflavin-sensitized photodynamic modification of collagen led to significant formation of cross-linked molecules. Sodium azide or l,4-diazabicyclo(2,2,2)octane, which are known to be singlet oxygen quenchers, and catalase could not inhibit the modification. Surprisingly, the collagen modification was accelerated in the presence of superoxide dismutase. The aggregation was accompanied by the loss of tyrosine and histidine residues in the collagen. An inhibitory effect of dissolved oxygen on the modification of collagen was observed. Similarly, the loss of tyrosine residues in the irradiated collagen was inhibited in the presence of dissolved oxygen. Dityrosine formation was also observed with the loss of tyrosine. These results indicate that photodynamic modification of tyrosine probably contributes to the riboflavin-sensitized cross-linking of collagen through the formation of dityrosine.Keywords
This publication has 33 references indexed in Scilit:
- Superoxide dismutase amplifies dye photosensitized production of desferal free radical: An electron spin resonance studyBiochemical and Biophysical Research Communications, 1989
- STUDIES ON THE in vitro O2‐DEPENDENT INACTIVATION OF NADH‐GLUTAMATE SYNTHASE FROM Chlamydomonas reinhardii STIMULATED BY FLAVINSPhotochemistry and Photobiology, 1987
- Charge transfer between tryptophan and tyrosine in proteinsBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1982
- Direct demonstration of electron transfer between tryptophan and tyrosine in proteinsBiochemical and Biophysical Research Communications, 1980
- Dityrosine in CollagenConnective Tissue Research, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Studies on Changes of Protein by Dye Sensitized PhotooxidationAgricultural and Biological Chemistry, 1970
- Dityrosine in a non-hydroxyproline, alkali-soluble protein isolated from chick aorta and bovine ligamentBiochemical and Biophysical Research Communications, 1969
- Formation of insoluble gels and dityrosine by the action of peroxidase on soluble collagensBiochemical and Biophysical Research Communications, 1968
- Evidence for dityrosine in elastinBiochemical and Biophysical Research Communications, 1967