Measurement of immunoreactive α-melanocyte-stimulating hormone in the blood of rainbow trout kept under various conditions

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Abstract
Initial attempts to measure α-MSH in trout blood by radioimmunoassay proved unsuccessful due to apparent losses of immunoreactive hormone, especially in the plasma of trout adapted to a black background. An extraction protocol for α-MSH from plasma is described. Subsequent radioimmunoassay revealed progressive increases in immunoreactive α-MSH as trout became adapted to a black background. Plasma α-MSH titres were raised during fungal infection, and also showed significant differences in fish obtained from different fish farms.