ROLE OF PHAGOCYTIC CELLS IN HUMAN-BLOOD LEUKOCYTE SUSPENSIONS FOR INVITRO COLONY-FORMING CELLS

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 6  (4) , 346-354
Abstract
The iron ingestion method was used to separate colony stimulating cells from in vitro [granulocytic] colony forming cells (CFU-C) in human white blood cell (WBC) suspensions. The depletion of phagocytic cells (granulocytes and monocytes) from WBC eliminated spontaneous colony growth, without influencing the total number of CFU-C. Phagocyte-free human WBC can be used as a target for measuring human colony stimulating activity (CSA). Such leukocytes can be cryopreserved, making possible the use of standardized target cell preparations for longitudinal studies. Hemolysate added to phagocyte-free blood leukocytes did not induce colony formation but could enhance it in the presence of a suitable CSA. Spontaneous colony formation of unseparated WBC was significantly reduced by selective destruction of granulocytes by freezing in 10% DMSO [dimethylsulfoxide], indicating that granulocytes and monocytes are involved in the production of endogenous CSA in WBC suspensions.