Identification of tubulin from the yeast Saccharomyces cerevisiae.

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Abstract
A tubulin-like protein was identified in the lower eukaryote S. cerevisiae. The criteria used were copolymerization of the 35S-labeled yeast protein with porcine brain tubulin, immunoprecipitation of the 35S-labeled yeast protein with antiflagellar [Chlamydomonas reinhardtii] tubulin antibody, the presence of the yeast protein as a constituent of isolated yeast nuclei and splitting of the yeast protein in a gel electrophoretic system containing sodium dodecyl sulfate that resolved the .alpha.- and .beta.-tubulin chains from other sources. This protein did not appear to have significant affinity for the plant alkaloid, colcemid.