Monosomics in Maize Induced by X-irradiation of the Pollen

Abstract
Five plants of maize having monosomic microsporocytes were isolated from the X1 following crosses of zb 4 Pwwbr 1 by irradiated pollen carrying Zb 4 PwrBr 1. Three plants were hemizygous for all 3 recessive markers in chromosome 1. Since the marked region contains the centromere and all dominant markers genes from the pollen parent were deficient, it was concluded that chromosome 1 was missing. Root-tips of 2 of the 3 plants were in division and were monosomic. Two other plants with 19 chromosomes in the pollen mother cells were also found. One plant had 19 chromosomes in the root-tips; mitosis was not obtained in the other. These 2 plants were not hemizygous for all 3 recessives in chromosome 1, suggesting that a different chromosome had been lost.

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