Inhibition of L5178Y Cells in Culture by Methotrexate and Antibiotics
Open Access
- 1 July 1976
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 10 (1) , 185-187
- https://doi.org/10.1128/aac.10.1.185
Abstract
Methotrexate, tetracycline, gentamicin, streptomycin, and penicillin inhibited the growth of L5178Y murine leukemia cells in culture with I 50 (concentration of drug that caused a 50% inhibition of growth at 72 h) values of 0.0028 μg/ml (6.2 × 10 −9 M), 7.9 μg/ml, 200 μg/ml, 1,700 μg/ml, and 3,000 μg/ml (5,000 U/ml), respectively. At concentrations achieved clinically or utilized in the laboratory, the antibiotics did not alter the I 50 of methotrexate.Keywords
This publication has 8 references indexed in Scilit:
- Effect of methionine deprivation on L5178Y murine leukemia cells in culture interference with the antineoplastic effect of methotrexateBiochemical Pharmacology, 1976
- Clinical Pharmacology of SisomicinAntimicrobial Agents and Chemotherapy, 1975
- Dependence of 5-methyltetrahydrofolate utilization by L5178Y murine leukemia cells in vitro on the presence of hydroxycobalamin and transcobalamin II.1973
- The effects of antibiotics and cancer chemotherapeutic agents on the cellular transport and antitumor activity of methotrexate in L1210 murine leukemia.1973
- Antibiotics Alter Methotrexate Metabolism and ExcretionScience, 1969
- Antibiotic inhibitors of the bacterial ribosome.1968
- DEVELOPMENT, MAINTENANCE AND ASSAY OF DRUG RESISTANCE.1964
- Increased levels of folic acid reductase as a mechanism of resistance to amethopterin in leukemic cellsBiochemical Pharmacology, 1961