Specific and reversible activation and inactivation of the mitochondrial phosphate carrier by cardiolipin and nonionic detergents, respectively

Publisher Website
Google Scholar
Abstract
The phosphate carrier of pig heart mitochondria was solubilized with Triton X‐100 and purified by chromatography on hydroxylapatite. Incubation of the phosphate carrier fraction with cardiolipin stimulated the reconstituted [32P]phosphate exchange activity in liposomes, whereas increased Triton X‐100 concentrations inhibited it. The effects of cardiolipin and Triton X‐100 are reversible. The activation by cardiolipin is highly specific and could not be obtained with any other applied phospholipid.