Severe Acute Respiratory Syndrome Coronavirus Protein 6 Is Required for Optimal Replication
- 1 March 2009
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 83 (5) , 2368-2373
- https://doi.org/10.1128/jvi.02371-08
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) encodes several accessory proteins of unknown function. One of these proteins, protein 6 (p6), which is encoded by ORF6, enhances virus replication when introduced into a heterologous murine coronavirus (mouse hepatitis virus [MHV]) but is not essential for optimal SARS-CoV replication after infection at a relatively high multiplicity of infection (MOI). Here, we reconcile these apparently conflicting results by showing that p6 enhances SARS-CoV replication to nearly the same extent as when expressed in the context of MHV if cells are infected at a low MOI and accelerates disease in mice transgenic for the human SARS-CoV receptor.Keywords
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