Use of gene fusions of the structural gene sdaA to purify L‐serine deaminase 1 from Escherichia coli K‐12
- 1 February 1993
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 211 (3) , 521-527
- https://doi.org/10.1111/j.1432-1033.1993.tb17578.x
Abstract
The purification by affinity chromatography of beta-galactosidase from strains carrying sdaA/lacZ gene fusions results in the copurification of L-serine deaminase 1. We conclude that sdaA is the structural gene for the latter enzyme. The purified L-serine deaminase 1 obtained after collagenase treatment of an sdaA-collagen-lacZ fusion differs from the native enzyme by the addition of several amino acids at the C-terminal. Like the enzyme in crude extracts, this purified enzyme is catalytically inactive, and is activated by incubation with iron and dithiothreitol.Keywords
This publication has 22 references indexed in Scilit:
- A rapid, high resolution DNA sequencing gel systemAnalytical Biochemistry, 1990
- A possible mechanism for the in vitro activation of L-serine deaminase activity in Escherichia coli K12Biochemistry and Cell Biology, 1990
- DNA bending is induced in an enhancer by the DNA-binding domain of the bovine papillomavirus E2 protein.Proceedings of the National Academy of Sciences, 1988
- Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.Journal of Biological Chemistry, 1987
- Nonenzymatic cleavage of proteins by reactive oxygen species generated by dithiothreitol and iron.Journal of Biological Chemistry, 1985
- Rapid purification of a cloned gene product by genetic fusion and site-specific proteolysis.Proceedings of the National Academy of Sciences, 1984
- One-step purification of hybrid proteins which have β-galactosidase activityGene, 1984
- Sequence determination and analysis of the 3' region of chicken Pro-.alpha.1(I) and Pro-.alpha.2(I) collagen messenger ribonucleic acids including the carboxy-terminal propeptide sequencesBiochemistry, 1981
- In vitro studies on L-serine deaminase activity of Escherichia coli K12Canadian Journal of Biochemistry, 1980
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977