Stereoselective synthesis and structure proof of a metabolite of vitamin D3, (23S,25R)-25-hydroxyvitamin D3 26,23-lactone (calcidiol lactone).

Abstract
Stereochemical configurations of biologically prepared 25-hydroxyvitamin D3 26, 23-lactone (calcidiol lactone) at C-23 and C-25 are determined to be S and R, respectively, by comparison of its high performance LC retention time with those of (23S, 25R)-and (23R, 25S)-25-hydroxyvitamin D3 26, 23-lactone which have been synthesized stereoselectively starting from C-22 steroid aldehyde and (R)-or (S)-citramalic acid.

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