Enzyme immunoassay method for comprehensive drug screening in micro-samples of urine.
Open Access
- 1 March 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 33 (3) , 367-371
- https://doi.org/10.1093/clinchem/33.3.367
Abstract
We adapted the reagents from 11 different enzyme-multiplied immunoassay technique (EMIT; Syva Co., Palo Alto, CA 94304) drug-detection kits for use in a centrifugal analyzer. The antibody reagents were mixed into a single dilute solution, and the enzyme-labeled drug derivatives were combined similarly (Mixed EMIT reagents), for use in testing urine samples for the presence of multiple drugs. The assay, a rapid comprehensive drug-screening technique, requires 100 microL of sample and is capable of testing seven samples, in duplicate, simultaneously, in less than 10 min. Clinical evaluation (n = 325) by comparison with thin-layer chromatography (TLC) had the following results: 230 samples were negative by TLC and Mixed EMIT, 77 samples were positive by TLC and Mixed EMIT, 16 samples were negative by TLC and positive by Mixed EMIT, and two samples were positive by TLC and negative by Mixed EMIT.This publication has 4 references indexed in Scilit:
- Urine Cannabinoid Analysis: An Integrated Multi-Method ApproachJournal of Analytical Toxicology, 1984
- Adaptation of EMIT® Procedures for Maximum Cost Effectiveness to Two Different Centrifugal Analyzer SystemsTherapeutic Drug Monitoring, 1981
- Monitoring Pediatric Serum Theophylline, Phenobarbital, and Phenytoin with the IL Multistat III®Therapeutic Drug Monitoring, 1980
- Assay of phenytoin: adaptation of "EMIT" to the centrifugal analyzer.Clinical Chemistry, 1976