A rapid and efficient method for DNA extraction from paraffin wax embedded tissue for PCR amplification
Open Access
- 1 June 1996
- journal article
- research article
- Published by BMJ in Molecular Pathology
- Vol. 49 (3) , M179-M180
- https://doi.org/10.1136/mp.49.3.m179
Abstract
DNA from archival, formaldehyde fixed, paraffin wax embedded human tissue, suitable for amplification by the polymerase chain reaction (PCR), was obtained using a microwave method based on the capture of DNA by magnetic beads. Fragments of the α-1-antitrypsin gene (AAT) and the apolipoprotein E gene (APOE) were amplified successfully from human liver and brain tissue, respectively. This procedure provides a more rapid, simple and efficient method for reproducibly obtaining DNA from preserved tissue that has been kept in storage for up to 30 years.Keywords
This publication has 6 references indexed in Scilit:
- Microwave-based DNA extraction from paraffin-embedded tissue for PCR amplification.1995
- Rapid preparation of tissue DNA from paraffin-embedded blocks and analysis by polymerase chain reaction.Journal of Histochemistry & Cytochemistry, 1993
- Apolipoprotein E genotyping by one-stage PCRThe Lancet, 1991
- Diagnosis of α1antitrypsin deficiency by enzymatic amplification of human genomic DNA and direct sequencing of polymerase chain reaction productsNucleic Acids Research, 1988
- Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction.The Journal of Experimental Medicine, 1988
- Analysis of DNA extracted from formalin-fixed, paraffin-embedded tissues by enzymatic amplification and hybridization with sequence-specific oligonucleotidesBiochemical and Biophysical Research Communications, 1987