Complementation of byrl in fission yeast by mammalian MAP kinase kinase requires coexpression of Raf kinase
- 1 July 1993
- journal article
- Published by Springer Nature in Nature
- Vol. 364 (6435) , 349-352
- https://doi.org/10.1038/364349a0
Abstract
Intracellular signalling from receptor tyrosine kinases in mammalian cells involves the activation of a signal cascade which includes p21ras and the protein kinases p74raf-1, MAP kinase kinase and MAP kinases. In the yeasts Schizosaccharomyces pombe and Saccharomyces cerevisiae the response to mating pheromones requires the Spk1 and KSS1/FUS3 kinases, which have sequence homology to vertebrate MAP kinases. The recent cloning of complementary DNAs for mammalian and frog MAP kinase kinases has shown that they are homologous to the S. pombe Byr1 (ref. 17) and S. cerevisiae STE7 (ref. 18) kinases, which have been proposed to function upstream of Spk1 and KSS1/FUS3, respectively. We have investigated whether these apparently similar kinase pathways are functionally conserved between vertebrates and S. pombe. We report here that expression of mammalian MAP kinase kinase alone fails to complement a byr1 mutant of S. pombe. When coexpressed with Raf kinase, however, MAP kinase kinase is activated by phosphorylation and the mating defect of the byr1 mutant is rescued. This suggests that the pathways are functionally homologous and that Raf kinase may directly phosphorylate and activate MAP kinase kinase.Keywords
This publication has 37 references indexed in Scilit:
- Activation of the MAP kinase pathway by the protein kinase rafCell, 1992
- Activation of Mitogen-Activated Protein Kinase Kinase by v-Raf in NIH 3T3 Cells and in VitroScience, 1992
- Raf-1 activates MAP kinase-kinaseNature, 1992
- Involvement of p21ras in activation of extracellular signal-regulated kinase 2Nature, 1992
- Ras is essential for nerve growth factor- and phorbol ester-induced tyrosine phosphorylation of MAP kinasesCell, 1992
- ras mediates nerve growth factor receptor modulation of three signal-transducing protein kinases: MAP kinase, Raf-1, and RSKCell, 1992
- FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction.Proceedings of the National Academy of Sciences, 1991
- Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases.Genes & Development, 1991
- FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugationCell, 1990
- A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiaeCell, 1989