The Effect of Ozone on Chloroplast Lamellae and Isolated Mesophyll Cells of Sensitive and Resistant Tobacco Selections

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Abstract
Ozone treatment of whole tobacco [Nicotiana tabacum] leaves affected chloroplast electron transport in proportion to the degree of visible leaf injury resulting on either the sensitive or resistant selection. When chloroplasts were isolated from Bel-W3 (ozone-sensitive) and Bel-B (ozone-resistant) tobacco leaves and exposed to identical ozone doses in vitro, however, inhibition of electron transport was similar in both. Likewise, enzymatically-isolated leaf mesophyll cells were equally sensitive to in vitro ozone treatment. The differential ozone response of Bel-W3 and Bel-B tobacco was not expressed by the isolated chloroplast lamellae or leaf mesophyll cells.